Olympus Fluoview FV1000 User Manual

Confocal Laser Scanning Biological MicroscopeFV1000FLUOVIEWFLUOVIEW—Always Evolving

Low Chromatic Aberration ObjectiveBest Reliability for Colocalization AnalysisA new high NA oil-immersion objective minimizes chromaticaberration in t

Technology / HardwareSwitchable between Confocal and TIRFM ImagingSwitchable between confocal and TIRFM imaging for localizationof proteins on the cyt

User-Friendly Software to Support Your Research.Time ControllerPrecisely synchronizes differentexperimental protocols including FRAP,FLIP and FRET by

Technology / HardwareRe-Use FunctionOpen previously configured scanningconditions and apply them to new orsubsequent experiments.Help GuideComprehensi

Broad Application Support and Sophisticated ExperimentControl.MeasurementLight StimulationMulti-Dimensional Time-LapseFRETColocalization3D MosaicImagi

Application15,000 20,000 30,00025,000Time (ms)35,000 40,0002004006008001,0001,2001,4001,6001,8002,0002,2002,400IntensityCH1CH2CH1CH2MeasurementDiffusi

This optional software module enables data acquisition and analysis to investigate the molecular interactionand concentrations by calculating the diff

Application/ Molecular Interaction Analysis0 µs0 ms0 ms1 ms2 ms3 ms4 msn ms10 µs 20 µs 30 µs 40 µs 50 µs n µsMolecule sizeSmall LargeSpatial Correlati

Laser Light StimulationThe SIM scanner system combines the main scanner with a laser light stimulation scanner.Control of the two independent beams en

Application/ Molecular Interaction AnalysisPhotoconversionThe Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent

1FLUOVIEW–—FromOlympus is OpenFLUOVIEW—More Advanced than EverThe Olympus FLUOVIEW FV1000 confocal laser scanning microscope deliversefficient and rel

Significantly Improved Long Time-Lapse ThroughputEquipped with motorized XY stage for repeated image acquisition from multiple points scattered across

Application/ Molecular Interaction Analysis3D Mosaic ImagingMosaic imaging is performed using a high-magnification objective to acquire continuous 3D

Expandability to Support Diverse Application.Application Standard Functions Optional FunctionsMolecular interaction and Intracellular diffusion measu

Expandability350350300300 400400 450450 500500 550550 600600 650650 700700 750750 800800405 LD440 LD473 LD635 LD559 LD458 Multi Argon515 Multi

Fluorescence Illumination UnitStand with Mercury lamp house, motorized shutter,and fiber delivery system for conventional fluorescenceobservation. Lig

ExpandabilityBCFluorescence illumination unit*Optional unitTransmitted light detection unitIX81IX81-ZDCInverted motorized microscopeBX61WI BX61Upright

Objectives NA W.D. (mm) DIC prism RevolvingnosepieceMPLN5X0.10 20.00 –WI-SSNP,WI-SRE3UMPLFLN10XW 0.30 3.50 WI-DIC10HRWI-SSNP,WI-SRE3UMPLFLN20XW 0.50

ExpandabilityDimensions (mm) Weight (kg) Power consumptionMicroscope with scan unit BX61/BX61WI 320 (W) x 580 (D) x 565 (H) 41—IX81 350 (W) x 750 (D)

FLUOVIEW websitewww.olympusfluoview.com• OLYMPUS CORPOARATION is ISO9001/ISO14001 certified.• Illumination devices for microscope have suggested lifet

2Imaging to Analysising up New WorldsFrom Imaging to AnalysisFV1000Advanced Deeper Imaging with High Resolution FV1000MPE

Advanced FLUOVIEW Systems Enhance the Power ofYour ResearchSuperb Optical Systems Set the Standard for Accuracy and Sensitivity.Two types of detectors

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Laser combiner/FiberDiode LaserGreater stability, longer service life andlower operating cost are achieved usingdiode lasers.Laser Feedback Control Sc

Technology / HardwareIX81PMTPMTPMTGalvanometerscanning mirrorsGalvanometerscanning mirrorsUIS2 objectivesSpecimenPupil projection lens SIM Scanner *Mi

Spectral Based DetectionFlexibility and High SensitivitySpectral detection using gratingsfor 2 nm wavelength resolutionand image acquisition matched t

Technology / HardwareSIM (Simultaneous) Scanner UnitCombines the main scanner with a dedicated laser lightstimulation scanner for investigating the tr