Olympus IX81 User Manual

INSTRUCTIONSIX81MOTORIZED INVERTEDRESEARCH MICROSCOPEA X 7 3 2 0This instruction manual is for the Olympus Motorized Inverted Research Microscope Mode

56. Restrictions in TV observation @The following combinations are not permitted in consideration of the optical performance. · IX2-SPT + PE4X + U-P

6IX81MODULE NOMENCLATURE}The modules shown below are only the representative modules. As there are other modules which can be combined withthe microsc

7 : Observation/ : Side portCONTROLS}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 49 to 58). · The illust

8IX81Allen screwdriverDiopter adjustmentring (Page 18)Other Modules}Same as the U-BI90CT except that the U-BI90does not have the CT turret and focus r

9Manipulator mountingscrew holesM6 screw (x 6)Y-axis knobX-axis knobCondenser height fineadjustment knob(Page 24)}This is a manually controlled conden

10IX81The button functions shown below are the initial setups for PC control (remote operation).The button functions can also be assigned as required

11TRANSMITTED LIGHT BRIGHTFIELDOBSERVATION PROCEDURE}The following flow shows the operating procedure for the transmitted light brightfield observatio

12IX81The button functions shown below are the initial setups at the moment the PC is started up.Controls with numbers inside are not available w

13Fig. 4USING THE CONTROLS4-1 Microscope Frame1 Voltage Indication(Fig. 4)}Set the main switch of the IX2-UCB control box to “ I ” (ON), then pres

14IX81Fig. 53 Magnification Change(Fig. 5)When the magnification selector knob 1 is pulled out, the magnificationwill be 1.6X. When the knob is pus

15Fig. 6Fig. 74-2 Focusing Block}The same effect as the focusing knob on the microscope frame can also be obtained using the U-FH focus adjustmentkno

16IX81Fig. 8Fig. 9Fig. 104-3 Stage1 Placing the Specimen(Figs. 8 to 10)With the IX2-SFR or IX-SVL2 Stage (Fig. 8)Place the specimen on the center

17Fig. 11Fig. 12Fig. 132 Moving the Specimen(Fig. 11)With the IX2-SFR or IX-SVL2 StageTo move the specimen to a desired position, rotate the X-axis

18IX81Fig. 14Fig. 15Fig. 16Fig. 174-4 Observation Tube1 Adjusting the Interpupillary Distance(Fig. 14)While looking through the eyepieces, adjust

19Fig. 18Fig. 19Fig. 204 Using Eyepiece Micrometer Disks(Fig. 18)Eyepiece micrometer disks can be inserted into the WHN10X-H(or WHN10X) eyepieces.U

20IX81Fig. 21Fig. 227 Adjusting the Tilt (U-TBI90 Only)(Fig. 21)}Adjust the height and tilt of the observation tube to obtain the most com-fortable

21Fig. 23Fig. 24Fig. 252 Mounting Filters(Figs. 23 to 25)}45 mm diameter, maximum 6 mm thick filters can be mounted. Variousfilters, such as the pr

22IX81Fig. 26Fig. 27Fig. 283 Using the Field Iris Diaphragm(Figs. 26 & 27)}The field iris diaphragm lever 1 is used to adjust the diameter of t

23Fig. 304-6 Condenser1 Centering the Condenser(Figs. 30 & 31)With the IX2-LWUCDA2/IX2-LWUCD Universal Condenser or IX-ULWCDCondenser1. Rotate

24IX81Fig. 31With the U-UCD8 Universal Condenser (Fig. 31)#For operation of the U-UCD8, refer to the instruction manualprovided with it.This condenser

IX81CONTENTS67-1011- 1213-27IMPORTANT — Be sure to read this section for safe use of the equipment. —1 MODULE NOMENCLATURE2 CONTROLS34 US

25Fig. 32Fig. 332 Using the Aperture Iris Diaphragm(Fig. 32)}In general, the potential resolving power of an objective is fully utilized if thediap

26IX81Fig. 34121 Oil-Proof Cap (UIS Series only)(Fig. 34)}By mounting the oil-proof cap (Type C1 or C2) on the tip of the applicableobjective, you

27Fig. 353 Using Immersion Objectives(Fig. 35)#Always use immersion oil supplied by Olympus.}If the objective in use can accommodate the oil-proof

28IX81OTHER OBSERVATION METHODS5-1 Phase Contrast Observation}A phase contrast objective, phase contrast optical element and the U-CT30 centering tel

29With the IX-ULWCD Condenser(Note) The IX-PHCU or IX-PH1U can be attached only in the Ph1 and PhC positions.Do not remove the built-in elements.Optic

30IX81Fig. 38Fig. 39Fig. 404. Hold a phase contrast ring and, while pushing the spring 5 insidethe turret with the edge of the phase contrast ring 6,

31Fig. 41Fig. 42Fig. 433 Centering the Phase Contrast Ring Slit(Figs. 41 to 43)}Before proceeding to the following, open the aperture iris diaphrag

32IX815-2 Differential Interference Contrast Observation}If a plastic petri dish is used, the normal optical performance of DIC observation cannot be

33CPlanFl 10X (IX-DP10) (IX-DP10) ––– –––LCPlanFl 20X (IX-DP20) (IX-DP20) (IX-DP20HC) (IX-DP20HR)40X IX-DP40 IX-DP40 IX-DP40HC IX-DP40HR60

34IX81Fig. 45Fig. 46Fig. 47Fig. 483 Attaching the Analyzer and DIC Slider(Figs. 45 to 47)With the U-DICT DIC Slider1. Remove the dummy slider from

35Fig. 49Fig. 50Fig. 515 Cross-Nicol Adjustment(Figs. 49 to 51)1. Rotate the condenser’s turret for the BF (brightfield) light path (with nooptical

36IX816 Observation Method1. Rotate the condenser turret to engage the suitable optical element forthe objective in use in the light path.2. Engage

37Fig. 525-3 Simplified Polarized Light Observation}An analyzer and polarizer are required for simplified polarized light observation. The applicable

38IX81Fig. 53PHOTOMICROGRAPHY AND TV OBSERVATION6-1 Photomicrography}Use the U-TR30H-2 trinocular observation tube or the side port for photomicrogra

39Fig. 56Fig. 57Fig. 583 Attaching the Photomicrographic system(Fig. 56) · Attach the photomicrographic system directly onto the photomicrographics

40IX816 Adjusting the IlluminationCorrect illumination is more crucial for photomicrography than for observation because flawless pictures cannot

41Fig. 596-2 TV Observation}The following four methods are available for TV observation.1. Attaching the video camera onto the side port2. Attaching

42IX813 Attaching the Camera on the Lower Back Port}A C-mount video camera can be used by ordering the manufacturer toinstall and adjust the IX2-LB

43TROUBLESHOOTING GUIDEUnder certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problemsocc

44IX81ProblemCause Remedy PageMagnification selector knob is in an in-termediate position.c) Field of view is obscured or notevenly illuminated.Turn t

IX815 OTHER OBSERVATION METHODS5-1 Phase Contrast Observation ...

45ProblemCause Remedy Pageg) Image is blurred. Objective is engaged incorrectly in thelight path.Make sure that revolving nosepiececlicks into place c

46IX81SPECIFICATIONSModuleSpecificationMicroscopeFrameIX81-S8F-3IX81-S1F-3IX81-F-3 · Motorized light path selection S8F-3: Side port 80% light pathS1F

47ObjectivesSpecificationsUIS2SeriesPlan achromatfor phasecontrastobservationCPlanN10XPh NA 0.25, WD 10.0 mm, ring slit IX-PHC or PHCUCPlanFLN10XPh NA

48IX81ModuleSpecificationOperating Environment · Indoor use · Altitude: Max. 2000 m · Ambient temperature: 5° to 40°C (41° to 104°F) · Maximum relati

49ASSEMBLY9-1 Assembly DiagramThe diagram below shows the sequence of assembly of the modules. The numbers indicate the order of assembly.The module

50IX81Fig. 60Fig. 619-2 Detailed Assembly Procedures#Be always sure to use the U-ZPCB(T2) Z-board.The following phenomena will occur if the U-ZPCB is

51Fig. 62Fig. 63Fig. 64Fig. 651 Mounting the Illumination Column(Fig. 62)1. Aligning the two guide holes on the illumination column with the twopro

52IX81Fig. 66Fig. 67Fig. 68Fig. 694 Attaching the Stage(Figs. 66 to 68)}The following stage mounting procedures are commonly applicable tothe IX2-S

53Fig. 70Fig. 71Fig. 72Fig. 73<< Attaching the Scales >>}Scales for use with a 96-well microtiter plate are provided with the me-chanical

54IX81Fig. 74Fig. 75Fig. 765 Attaching the Objectives(Fig. 74)1. Remove the stage center plate and attach the objectives to the revolvingnosepiece

1IMPORTANTThe IX81 microscope can be used in stand-alone mode (operated from the U-HSTR2 hand switch) or can operated from aPC when the IX2-BSW softwa

55Fig. 77Fig. 78Universal Condenser (U-UCD8) (Figs. 77 & 78)<< Attaching onto the UCD Adapter >>1. Using the Allen screwdriver, loosen

56IX81Fig. 79Fig. 80Fig. 81Fig. 827 Attaching the Observation Tube(Figs. 79 to 82)Binocular Tube (U-BI90CT, U-BI90, U-TBI90) (Figs. 79 & 80)1.

57Fig. 83Fig. 848 Attaching the Eyepieces(Fig. 83)1. Remove the eyepieces’ dust caps.2. Insert the WHN10X-H eyepiece with helicoid into the eyepiec

58IX81Rubber foot seatsFig. 85Fig. 869}The microscope frame clamping method is variable depending on theframe stabilizing feet on the rear of the fram

59 PROPER SELECTION OF THE POWER SUPPLY CORDIf no power supply cord is provided, please select the proper power supply cord for the equipment by r

60IX81Table 2 HAR Flexible CordAPPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODSApproval OrganizationPrinted or Embossed Harmoniza-tio

6110LAMP HOUSING INSPECTION SHEETz Study the instruction manual for the lamp housing before inspection.z For safe use of the lamp housing, we recommen

Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, JapanWendenstraße 14-18, 20097 Hamburg, Germany3500 Corporate Parkway, P.O. Box 61

2IX811. After the equipment has been used in an observation of a specimen thatis accompanied with a potential of infection, clean the parts coming inc

3Fig. 2125. The microscope is provided with a simplified waterproof mechanism.Therefore, if culture liquid or water is spilt on the stage, revolving n

4IX81Safety SymbolsSymbol ExplanationIndicates that the surface becomes hot, and should not be touched with bare hands.Indicates that the main switch