Olympus IX2-DSU BX-DSU User Manual

DISK SCANNING BIOLOGICAL MICROSCOPEPetitionThank you for your adoption of OLYMPUS microscope, this time.Please read this manual carefully in order to

INTRODUCTION4PageWarning labelsWarning label is attached to the place where caution is particularly required for operation. Observe theinstructions.La

INTRODUCTION5PageWhen using the systeml Handle this system with care so as not to give a shock as it is a precision instrument.l This system require

INTRODUCTION6PageManual conventionsThis manual describes with use of the following manual conventions.² Conventions of note, reference and pointConve

II..

SYSTEM OUTLINE / PrincipleI. System OutlineI. 1-1Page1 SYSTEM OUTLINEAttaching this system to a microscope, it comprises a confocal microscope system

SYSTEM OUTLINE / FeaturesI. System OutlineI. 1-2Page1-2 Features● Confocal disk employed enables to acquire image of confocal effect. ● As while lig

SYSTEM OUTLINE / System ConfigurationI. System OutlineI. 1-3Page1-3 System Configuration1-3-1 In case of BX seriesFigure of BX series System Configura

SYSTEM OUTLINE / System ConfigurationI. System OutlineI. 1-4Page Table of Instruction Manuals for Modules to be integratedInstruction Manual Modul

SYSTEM OUTLINE / System ConfigurationI. System OutlineI. 1-5Page1-3-3 In case of IX seriesFigure of IX Series System ConfigurationWhen you use this sy

SYSTEM OUTLINE / System ConfigurationI. System OutlineI. 1-6Page Table of Instruction Manuals for Modules to be integratedInstruction Manual Modul

IIII..

SUMMARY OF OBSERVATION PROCEDURESII . DSUII . 2-1Page2 SUMMARY OF OBSERVATIONPROCEDURESThis section describes procedures for fluorescent observation

Summary of observation proceduresII . DSUII . 2-2Page(From previous page)Turn turret of rear side and put camera mirror unitthat suits to the specimen

DETAILS OF EACH OPERATION / General Precaution for observationII . DSUII . 3-1Page3 DETAILS OF EACH OPERATION3-1 General Precaution for observation1

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unitII . DSUII . 3-2PageBe careful that specimen of certain kind cannot use anti-colorde

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unitII . DSUII . 3-3PageTable of combination for mirror unit and dichroic mirror filterE

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unitII . DSUII . 3-4PageColor Separation Filter CombinationsExcitationMethodMirror Unit

DETAILS OF EACH OPERATION / Table of Combination – Objective lens to each observation and applicablediskII . DSUII . 3-5Page3-3 Table of Combination

CONTENTSCorrect assembly and adjustments are critical for this system to manifest its full performance. If you are going toassemble the microscope you

DETAILS OF EACH OPERATION / Table of Combination – Objective lens to each observation and applicablediskII . DSUII . 3-6PageReflected light fluoresc

DETAILS OF EACH OPERATION / Assignment of operating buttons on body (BX61, BX62 or BX61WI only)II . DSUII . 3-7Page3-4 Assignment of operating butto

DETAILS OF EACH OPERATION / Powering upII . DSUII . 3-8Page*1In case that control box (IX2-UCB) is of type 1, the identifying plate indicates IX2-UC

DETAILS OF EACH OPERATION / Changeover of visual mirror unit (front side)II . DSUII . 3-9Page3-7 Changeover of visual mirror unit (front side)3-7-1

DETAILS OF EACH OPERATION / Disk IN/OUTII . DSUII . 3-10Page3-8 Disk IN/OUTWhen you wish to insert disk into light path, press left IN button of DSU

DETAILS OF EACH OPERATION / Changeover of camera mirror unit (rear side)II . DSUII . 3-11Page3-10 Changeover of camera mirror unit (rear side)Press

DETAILS OF EACH OPERATION / Adjustment of field iris diaphragmII . DSUII . 3-12Page3-12 Adjustment of field iris diaphragm1. Turn front side turret

DETAILS OF EACH OPERATION / Lamp centeringII . DSUII . 3-13Page3-13 Lamp centeringLamp centering should be done after arc image of lamp isstablized

DETAILS OF EACH OPERATION / Lamp centeringII . DSUII . 3-14Page11. Turn lamp centering knob (4) and overlap arc image andmirror arc image. (D)When

DETAILS OF EACH OPERATION / Camera focus alignmentII . DSUII . 3-15Page3-14 Camera focus alignment1. Bring a live image on monitor. Put objectiv

CONTENTSII. DSU2 SUMMARY OF OBSERVATION PROCEDURES ... 2-13 DETAILS OF EACH OPERATION... 3-13-1 General Precaution f

VARIETIES OF OBSERVATION METHODS / Visual observation (BX)II . DSUII . 4-1Page4 VARIETIES OF OBSERVATIONMETHODS4-1 Visual observation (BX)This syste

VARIETIES OF OBSERVATION METHODS / Visual observation (BX)II . DSUII . 4-2Page4-1-2 Simultaneous observation of reflected-light fluorescent and tran

VARIETIES OF OBSERVATION METHODS / Visual observation (BX)II . DSUII . 4-3PageWhen change of reflected-light fluorescence and transmitting Nomarskid

VARIETIES OF OBSERVATION METHODS / Visual observation (IX)II . DSUII . 4-4Page4-2 Visual observation (IX)This system can be used for not only reflec

VARIETIES OF OBSERVATION METHODS / Visual observation (IX)II . DSUII . 4-5Page4-2-2 Simultaneous observation of reflected-light fluorescence and dif

VARIETIES OF OBSERVATION METHODS / Camera observation (BX-IX)II . DSUII . 4-6Page4-3 Camera observation (BX-IX)This system provides reflected-light

TROUBLESHOOTING GUIDEII . DSUII . 5-1Page5 TROUBLESHOOTING GUIDEDepending upon how the system is used, it may not exhibit adequate performance of th

TROUBLESHOOTING GUIDEII . DSUII . 5-2PageTrouble Cause Countermeasure RefFilter like that is enteringlight path.Displace unnecessary substance from

TROUBLESHOOTING GUIDEII . DSUII . 5-3PageTrouble Cause Countermeasures Ref.Ambient light (such asfluorescent lamp) isentering light path.Turn off fl

CONTENTS4-1-2 Simultaneous observation of reflected-light fluorescent and transmittingNomarski differential interference...

SPECTRAL CHARACTERISTICS OF FILTERSII . DSUII . 6-1Page6 SPECTRAL CHARACTERISTICS OFFILTERSRFPHQ DSU-MRFPHQ0%100%300 400 500 600 700 800Wavelength (

SPECIFICATIONS / BX SeriesII . DSUII . 7-1Page7 SPECIFICATIONS7-1 BX SeriesItem SpecificationsLens barrel magnification 1xNumber of views Visual ob

SPECIFICATIONS / IX SeriesII . DSUII . 7-2Page7-2 IX SeriesItem SpecificationsLens-barrel magnification 1xNumber of views Visual observation side:

ASSEMBLY / System block diagramII . DSUII . 8-1Page8 ASSEMBLY8-1 System block diagram8-1-1 In case of BXMicroscopeBX51/BX52/BX61/BX62/BX51WI/BX61WIM

ASSEMBLY / System block diagramII . DSUII . 8-2Page8-1-2 In case of IXMotorizedFilter WheelU-FWRDSU Disk BoxDU-DBIXMercurylamp orXenon lampHalogenLa

ASSEMBLY / Installation of light illuminatorII . DSUII . 8-3Page8-2 Installation of light illuminator8-2-1 In case of BX1. Remove caps of mounting

ASSEMBLY / Installation of disk boxII . DSUII . 8-4Page8-2-2 In case of IX1. Loosen fixing screws (1) (2 places) that fix lightilluminator to micro

ASSEMBLY / Removal of transportation lockII . DSUII . 8-5Page8-4 Removal of transportation lockThis work should be done before connectingcable of di

CONTENTS8-9 Installation of front side turret... 8-108-9-1 In case of BX ...

ASSEMBLY / Installation of illuminatorII . DSUII . 8-6Page8-5 Installation of illuminator1. Loosen fixing screws (1) (2 places) that fix disk box t

ASSEMBLY / Installation of mercury lampII . DSUII . 8-7Page8-7 Installation of mercury lamp1. Loosen socket fixing screw (1), using the Allen screw

ASSEMBLY / Installation of mercury lampII . DSUII . 8-8PageLife meter setting1. Reset button (1) is located at front panel. Press center part (2)

ASSEMBLY / Lamp power supply settingII . DSUII . 8-9Page8-8 Lamp power supply settingCord or cable is weak against bend or twist. Care must betaken

ASSEMBLY / Installation of front side turretII . DSUII . 8-10Page8-9 Installation of front side turret8-9-1 In case of BX1. (Removal of turret). Lo

ASSEMBLY / Installation of visual mirror unit (front side)II . DSUII . 8-11Page8-10 Installation of visual mirror unit (front side)8-10-1 In case of

ASSEMBLY / Installation of visual mirror unit (front side)II . DSUII . 8-12Page8-10-2 In case of IX1. Remove cover (1) that is sucked with magnet f

ASSEMBLY / Installation of visual mirror unit (front side)II . DSUII . 8-13Page8-10-3 Method to make optionnal fluorescent mirror unitYou can make o

ASSEMBLY / IN/OUT of rear side turretII . DSUII . 8-14Page8-11 IN/OUT of rear side turretWhen you install or detach the turret, you must do the work

ASSEMBLY / Disk installation and changeII . DSUII . 8-15PageFor camera observation, use fluorescent mirrorunit of HQ type.The fluorescent mirror uni

INTRODUCTION1PageINTRODUCTION- Be sure to read this section for safe use of the equipment. -CautionThis manual – part or whole, shall not be used or r

ASSEMBLY / Installation of light shielding tube (BX61, BX62, BX51 or BX52)II . DSUII . 8-16Page6. Set the disk, putting the side attached with name

ASSEMBLY / Installation of UV-cut plateII . DSUII . 8-17Page8-15 Installation of UV-cut plate8-15-1 In case of BX1. Put UV-cut plate underneath the

ASSEMBLY / Filter IN/OUT inside motorized filter wheelII . DSUII . 8-18Page8-17 Filter IN/OUT inside motorized filter wheelAt ex-factory, filters ar

ASSEMBLY / Field iris diaphragm IN/OUTII . DSUII . 8-19Page4. Insert presser ring driver (4) into slots (5) of presser ring and turn it inclockwise

ASSEMBLY / Installation of camera adapter and filterII . DSUII . 8-20Page8-19 Installation of camera adapter and filter8-19-1 In case of DSU C-mount

ASSEMBLY / Installation of camera adapter and filterII . DSUII . 8-21Page5. Loosen fixing screw (7) of disk box, using the Allen screwdriver.6. In

ASSEMBLY / Installation of cameraII . DSUII . 8-22Page8-20 Installation of camera1. Loosen fixing screw (1) of port, using the Allen screwdriver th

ASSEMBLY / DIP switch settings on control box (BX61, BX62 and BX61W1 only)II . DSUII . 8-23Page8-21 DIP switch settings on control box (BX61, BX62 a

ASSEMBLY / Z board setting and installationII . DSUII . 8-24Page8-23 Z board setting and installation8-23-1 In case of BX61 and BX62For Z board, use

ASSEMBLY / Z board setting and installationII . DSUII . 8-25Page3. Using knobs you removed, fix Z board (2). Likewise, attach the othercover (3).Ke

INTRODUCTION2Page3. Loosen knob (5) of disk unit (at 2 places) and open disk protectivecovers (6).4. Loosen transportation lock knob (7) and remove

ASSEMBLY / Z board setting and installationII . DSUII . 8-26PageZ board installationYou must install Z board after you turn power of control boxto O

ASSEMBLY / Z board setting and installationII . DSUII . 8-27PageSetting change of ONBOARD DIP SWUse DIP SW set to OFF except the following DIP SW.Tu

OLYMPUS CORPORATION2-43-2,Hatagaya, Shibuya-ku, Tokyo, JapanOLYMPUS OPTICAL CO.(EUROPA) GMBH.Postfach 10 49 08, 20034, Hamburg, GermanyOLYMPUS AMERICA

INTRODUCTION3Pagel Do not turn the lamp ON without installing lamp house to microscope. Ultraviolet ray of lamp is hazardous toyour eyes. Do not l