Olympus IX81 User Manual download pdf (Page 30)

Fig. 32

Fig. 33

Using the Aperture Iris Diaphragm

(Fig. 32)

}In general, the potential resolving power of an objective is fully utilized if the

diaphragm is stopped down to correspond with the numerical aperture

(N.A.) of the objective.

}Depending on the specimen, image contras or focal depth in observa-

tion or photomicrography may be improved by keeping the aperture iris

diaphragm stopped down a little.

In general. a good image is obtained if the diaphragm is stopped down

to between 70% and 80% of the N.A. of the objective. Stop further down

for less contrasty specimens.

}To check the position of the perimeter of the aperture iris diaphragm,

remove the eyepieces and look into the eyepiece sleeves to view the

aperture iris diaphragm image and the objective’s exit pupil.

}When using the U-BI90CT binocular observation tube, the aperture iris

diaphragm can be observed by setting the turret to position “CT” or using

the U-CT30 centering telescope.

Flipping Up the Condenser Holder

(Fig. 33)

}To create working space and facilitate specimen replacement, microma-

nipulator positioning or mounting an objective through the hole on the

stage, flip up the condenser holder upwards.

1. Placing a hand against the bottom of the condenser holder, press it

upwards.

2. The tension of the flipping-up operation can be adjusted by turning the

adjustment screw 1 using a coin.

#Adjust the tension so that the flipped-up condenser will not drop

down by itself.

#When returning a flipped-up condenser to its original position, do so

gently and slowly.

Unless the condenser is returned to the correct position, optimum

illumination performance will not be achieved.

70-80%

30-20%

Objective pupil

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Olympus IX81 User Manual Page 30