Olympus Fv300 User Manual Page 1

CONFOCAL LASER SCANNINGBIOLOGICAL MICROSCOPESFV300FLUOVIEW

9Tile displayTopographic projectionHeight of 3D structure indicated by color.X-ZY-Z X-Y-ZX -ZY -ZUsing multiple 3D images to obtain accurate 3D struct

10Link with patch clamping data• With PCs linked through a LAN, Physiolinkcan synchronize electrophysiological andconfocal image data simultaneously.•

11Lucifer Yellow: retina ganglion cellTexasRed: dopamine-operated amacrine cellProf. Shigetada NakanishiDept. of Biological Sciences, Kyoto Univ. Facu

12Fluorescent ProteinsC elegans expressing beta-integrin fused to GFPDr. Xioping Xhu and Dr. John PlenefischUniversity of Toledo, Dept. of BiologyGFP-

Fluorescence Dyes and Filters300400500600700BA530RIFBA550RIFBA650RIFBA430-460BA465-495BA480-495BA470-520BA510-540BA520-550BA505-550BA505-525BA480-510B

Objectives for fixed stage uprightmicroscopes (using WI-UCD, WI-DICTHRA)14Item SpecificationsLaser light Visible light laser source Select from the f

● All brands are trademarks or registered trademarks of their respective owners.● Monitor images are simulated.Specifications are subject to change wi

1FV300 CONFOCAL LASER SCANNING MICROSCOPERFORMANCE FOR THE PERSONAL USERThe FV300 is the ideal choice of laser scanning microscopes for personal users

2OPEqertyuio!0!1!2wq Optical fiber for laser introductionw Beam collimatore Polarizerr Dichromatic mirrort Excitation dichromatic mirrory XY galvanome

Preset the conditions for imageacquisition and loadingStorage of Acquisition Settings enablesimmediate, one-touch recall of all therelevant experiment

Image tool barX-Y-Z scanning operations and time-lapse observations both produce multipleimages, which can be displayed insequence simply by clicking

5X -tSlantLine-tFreeLine-tY -tY-Z-tX-Y-tZoomIn-tX-Z-tX-Y-tX-Y-Z-tZoomIn-Z-tPatch clampBX61WI fixed stage upright microscope+translation stageImage acq

6The 440nm diode laser can be addedfor CFP/YFP FRET imaging.A 440nm diode laser is optionally availablefor CFP/YFP imaging. The 440nm laser lineideall

7PAPP for FRAP ApplicationPAPP: Programmable Acquisition Protocol ProcessorEasy, reliable flow of experiments for fluorescence recovery after photoble

8Multi-point time lapse systemHigh-magnification multi-point time lapse observation of living cells.Wide-ranging specimen observationsfor improved exp