Olympus Fv300 User Manual download pdf (Page 6)

X -t

SlantLine-t

FreeLine-t

Y -t

Y-Z-tX-Y-t

ZoomIn-t

X-Z-t

X-Y-t

X-Y-Z-t

ZoomIn-Z-t

Patch clamp

BX61WI fixed stage upright microscope+translation stage

Image acquisition ROI designation Intensity versus time

measurement

Calcium sparks in isolated cardiac myocyte

Dr. Sandor Gyorke

Texas Technical University

X -t

Time Course

Using different scanning modes to chart time-lapse changes efficiently.

High-speed (4 frames/sec) image

acquisition

For the high speed observation of the

sample, Fluoview is capable of scanning 4

frames per second in a fast scanning mode

at an image size of 512X512. By limiting the

image size, the frame rate will be even

faster. This scanning mode is suitable for

living cell observation.

Versatile line scanning modes have

many uses

The wide variety of the line scanning modes

(linear/slant/free-line) enables flexible

analysis of rapid time-lapse experiments.

Superior slice patching system

In combination with the unique fixed stage &

nosepiece focusing BX61WI microscope,

the FV300 provides a highly effective

system for slice patching. This unique set-

up has a small footprint for increased room

in a space-limited cage. The remote control

microscope options minimize the danger of

accidentally touching the delicate

experimental settings.Olympus also offers

ideal non-cover glass long working distance

water immersion objectives and an optional

XY translation stage that moves the entire

confocal microscope system while the

sample and other experimental hardware

remains in a fixed position.

Highly precise time-lapse analysis

Fluoview’s wide dynamic range of 12-bit or

4096 grey levels provides enough sensitivity

to detect even the slightest changes in

intensity. The user can designate multiple

regions of interest (ROI) by using drawing

tools. The fluorescence intensity or the ratio

can be analyzed with the intuitive GUI driven

program.

Calcium wave in Xenopus oocyte, Calcium Green staining, fluorescence

pseudo-colored fluorescence image after injection of inositol 3-trisphospate

Japan Science and Technology Corporation, Exploratory Research for Advanced Technology,

Mikoshiba cell control project, Prof. Aya Muto

Calcium wave in isolated cardiac myocyte

Dr. Sandor Gyorke

Texas Technical University

Long working distance, non-cover

glass water immersion objective

Immersion-type LUMPLFL objectives

The 40X water immersion objective in this series has a

3.3mm working distance and an extremely fine tip which

is suitable for micromanipulation using a fixed stage

upright microscope. It has a large N.A. (0.8) and is also

ideal for confocal observations. When using the BX61WI

fixed stage & nosepiece focusing upright microscope

with water immersion objectives, confocal imaging can

be used to monitor time-lapse fluorescence changes in

thick specimens such as brain slices.

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Olympus Fv300 User Manual Page 6

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