Olympus Fv300 User Manual download pdf (Page 11)

Link with patch clamping data

• With PCs linked through a LAN, Physiolink

can synchronize electrophysiological and

confocal image data simultaneously.

• With the same time stamp recorded in the

two PCs, it is possible to access and

analyze an image and its patch clamping

data corresponding to the Physiolink

software time scale.

• The patch clamping graph and Physiolink

software are interlocked and activated

concurrently.

• Physiolink software complies with the

FV300’s high-speed scanning, enabling

msec analysis.

Connecting two PCs by LAN is required.

Analyzing the degree of intensity overlap between channels.

Colocalization

Analyzing the state of a cell interior by synchronizing electrophysiological and confocal

image data. (

Optional software).

Physiolink

FV300

Ethernet

Axon

Clampex

Physioview software (Axon PC side)

Working conditions: Clampex Ver. 8.0 or later

Physiolink software (FV300 side)

Colocalization image (white)

Colocalization

By using this function to analyze multi-color

specimens, it is possible to discover

whether different labeled substances are

present in the same region. The ability to

quantify the Pearson correlation, the

overlapping coefficient and the

colocalization index allows colocalization

volumes to be compared between different

specimens. Images can also be analyzed in

series.

Thresholds Mode Regions/

Min-Max Bound Mode

Pacemaker neuron: Sea-slug (nudibranch)

Dr. Stuart Thompson, Department of Biological Sciences, Hopkins Marine Station, Stanford University.

Threshold Mode

Threshold lines can be interactively altered.

Regions/Min-Max Mode

Setting the ROI (region of interest) on the histogram makes it possible to create a

colocalization image. Values can also be obtained for Pearson correlation, overlapping

coefficient and colocalization index.

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Olympus Fv300 User Manual Page 11

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